[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"health-study-detail:100599452":3},{"organization":4,"outcomesModule":7,"designInfo":68,"detailedDescription":67,"studyPopulation":67,"armGroups":74,"interventions":81,"overallOfficials":67,"centralContacts":87,"locations":93,"responsibleParty":109,"collaborators":67,"id":113,"slug":67,"hasResults":114,"nctId":115,"briefTitle":116,"officialTitle":117,"acronym":118,"eligibilityCriteria":119,"healthyVolunteers":120,"sex":121,"minAge":122,"maxAge":123,"enrollmentInfo":124,"targetDuration":67,"studyType":127,"phases":128,"briefSummary":129,"conditions":130,"keywords":132,"overallStatus":95,"whyStopped":67,"lastUpdateSubmitDate":135,"lastUpdatePostDateStruct":136,"startDateStruct":139,"completionDateStruct":141,"leadSponsor":143,"locationsCount":144},{"fullName":5,"class":6},"Texas Tech University","OTHER",{"primaryOutcomes":8,"secondaryOutcomes":57,"otherOutcomes":67},[9,13,16,20,23,26,29,33,36,39,42,45,48,51,54],{"measure":10,"description":11,"timeFrame":12},"Fasting blood glucose (mg\u002FdL)","Fasting blood glucose will be measured via fingerstick at each testing visit. Values will be used to examine changes in glucose regulation in response to hydration status across the HDR Protocol phases.","Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)",{"measure":14,"description":15,"timeFrame":12},"Total body water (L)","Bioelectrical impedance spectroscopy (BIS) will be used to assess changes in total body water, BIS measurements will be collected at each testing visit to evaluate fluid shifts.",{"measure":17,"description":18,"timeFrame":19},"Intracellular water (L)","Description: Bioelectrical impedance spectroscopy (BIS) will be used to assess changes in intracellular water across hydration phases. BIS measurements will be collected at each testing visit to evaluate fluid shifts.","Time Frame: Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing)",{"measure":21,"description":22,"timeFrame":19},"Extracellular water (L)","Description: Bioelectrical impedance spectroscopy (BIS) will be used to assess changes in extracellular water across hydration phases. BIS measurements will be collected at each testing visit to evaluate fluid shifts.",{"measure":24,"description":25,"timeFrame":12},"Hand grip strength (kg)","Handgrip strength will be measured using a handheld dynamometer at four time points during the HDR Protocol. Measurements will be used to assess the impact of hydration status on upper body muscular strength.",{"measure":27,"description":28,"timeFrame":12},"Isometric leg pull strength (kg)","Isometric lower body strength will be evaluated using a standardized leg pull test at four timepoints during the HDR Protocol. Results will be used to determine the effect of hydration status on lower body muscular strength.",{"measure":30,"description":31,"timeFrame":32},"Total sleep time (hrs)","Total sleep time will be continuously monitored throughout the HDR Protocol using a wearable device.","through study completion, 18 days",{"measure":34,"description":35,"timeFrame":32},"Sleep efficiency (%)","Sleep efficiency will be continuously monitored throughout the HDR Protocol using a wearable device.",{"measure":37,"description":38,"timeFrame":32},"Number of awakenings (count)","Number of awakenings will be continuously monitored throughout the HDR Protocol using a wearable device.",{"measure":40,"description":41,"timeFrame":12},"PBMC H2O2 ( µg\u002FmL)","Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. H2O2 will be assessed.",{"measure":43,"description":44,"timeFrame":12},"DRP1 (Arbitrary Units)","Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Fission expression (DRP1).",{"measure":46,"description":47,"timeFrame":12},"MFN (Arbitrary Units)","Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Mitochondrial fusion expression (MFN) will be assessed.",{"measure":49,"description":50,"timeFrame":12},"PINK1 (Arbitrary Units)","Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Mitophagy marker (PINK1) will be assessed.",{"measure":52,"description":53,"timeFrame":12},"Parkin (Arbitrary Units)","Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Mitophagy marker (Parkin) will be assessed.",{"measure":55,"description":56,"timeFrame":12},"Malondialdehyde (nmol\u002Fmg)","Peripheral blood mononuclear cells (PBMCs) will be isolated from whole blood samples collected at four time points during the HDR Protocol. Lipid peroxidation will be assessed by measuring malondialdehyde concentrations.",[58,61,64],{"measure":59,"description":60,"timeFrame":12},"Change in hydration status via urine specific gravity (USG)","Hydration status will be assessed using urine specific gravity (USG) at four time points across the HDR Protocol: prior to the hydration phase, following the hydration phase, after the dehydration phase, and after the rehydration phase. These values will be used to confirm the effectiveness of each fluid manipulation phase.",{"measure":62,"description":63,"timeFrame":12},"Total lean mass (g)","Body composition will be assessed using dual-energy X-ray absorptiometry (DXA) at four time points across the HDR Protocol. Total lean mass will be evaluated in relation to hydration status at each phase.",{"measure":65,"description":66,"timeFrame":12},"Total fat mass (g)","Body composition will be assessed using dual-energy X-ray absorptiometry (DXA) at four time points across the HDR Protocol. Total fat mass will be evaluated in relation to hydration status during each phase.",null,{"allocation":69,"interventionModel":70,"interventionModelDescription":67,"primaryPurpose":71,"observationalModel":67,"timePerspective":67,"maskingInfo":72},"NA","SINGLE_GROUP","PREVENTION",{"masking":73,"maskingDescription":67,"whoMasked":67},"NONE",[75],{"label":76,"type":77,"description":78,"interventionNames":79},"Hydration-Dehydration-Rehydration (HDR) Protocol Arm","EXPERIMENTAL","Participants in this arm complete the HDR Protocol over 12 days and attend five lab visits.",[80],"Behavioral: Hydration-Dehydration-Rehydration Protocol (HDR)",[82],{"type":83,"name":84,"description":85,"armGroupLabels":86,"otherNames":67},"BEHAVIORAL","Hydration-Dehydration-Rehydration Protocol (HDR)","The HDR Protocol is a 12-day, sequential fluid manipulation intervention designed to assess the physiological effects of controlled changes in hydration status. The protocol consists of three consecutive 4-day phases:\n\nHydration Phase (Days 1-4): Participants follow a standardized fluid intake protocol designed to promote and maintain a euhydrated state. Hydration status is monitored using objective measures such as urine specific gravity and body mass.\n\nDehydration Phase (Days 5-8): Participants adhere to a fluid-restriction regimen to induce a hypohydrated state. The level of dehydration is verified through the same hydration status measures to ensure consistent and safe hypohydration.\n\nRehydration Phase (Days 9-12): Participants resume the standardized fluid intake protocol used during the Hydration Phase to restore euhydration. Rehydration effectiveness is confirmed through repeated hydration assessments.",[76],[88],{"name":89,"role":90,"phone":91,"phoneExt":67,"email":92},"Hui Ying Luk, Ph.D.","CONTACT","806-834-0827","huiying.luk@ttu.edu",[94],{"facility":5,"status":95,"city":96,"state":97,"zip":98,"country":99,"countryCode":100,"cosmosGeoPoint":101,"geoPoint":106,"contacts":107},"RECRUITING","Lubbock","Texas","79409","United States","US",{"type":102,"coordinates":103},"Point",[104,105],-101.85517,33.57786,{"lat":105,"lon":104},[108],{"name":89,"role":90,"phone":91,"phoneExt":67,"email":92},{"type":110,"investigatorFullName":111,"investigatorTitle":112,"investigatorAffiliation":5,"oldNameTitle":67,"oldOrganization":67},"PRINCIPAL_INVESTIGATOR","Hui Ying Luk","Assistant Professor","100599452",false,"NCT07084675","Dehydration, Rehydration, and Impact on Physiology","Impact of Mild Dehydration and Subsequent Rehydration on Cellular Stress and Physiological Performance","DRIP","Inclusion Criteria:\n\n* Apparently Healthy\n* BMI \\\u003C 30 kg\u002Fm2\n* Body weight is at least 110 lbs\n\nExclusion Criteria:\n\n* Currently pregnant\n* Have Type 1 Diabetes or Type 2 Diabetes\n* Have chronic kidney disease or a history of kidney stones\n* Have hypertension\n* Take medications that can cause fluid retention, such as corticosteroids, chronic use of Nonsteroidal Anti-inflammatory Drugs (NSAIDs), Hormonal Therapies (e.g., estrogen, androgens or anabolic steroids), Antihypertensives, Psychiatric Medications (e.g., Lithium), Vasodilators\n* Take medications that reduce fluid retention: diuretics, and RAAS Inhibitors (e.g., ACE inhibitors and ARBs)",true,"ALL","45 Years","65 Years",{"count":125,"type":126},15,"ESTIMATED","INTERVENTIONAL",[69],"Our goal is to determine whether hydration status influences inflammation, and overall physiological performance in adults aged 45-65.\n\nAim 1: The effects of 4-day mild dehydration followed by 4-day rehydration (defined as approximately 2-3% body mass loss) on H2O2, upstream inflammatory signal (NFkB), mitochondrial fragmentation, and autophagy in circulating PBMCs.\n\nAim 2: The effects of 4-day mild dehydration followed by 4-day rehydration (defined as approximately 2-3% body mass loss) on fasting glucose, sleep performance, and strength performance.\n\nParticipant will:\n\ncomplete a 4-day hydration protocol, followed by a 4-day dehydration protocol, and then a 4-day rehydration protocol.\n\nattend five laboratory visits for testing: Day 1: Informed consent and familiarization Day 2: Pre-hydration testing (before the 4-day hydration protocol) Day 3: Pre-dehydration testing (before the dehyration protocol) Day 4: Post-dehydration testing (after the dehydration protocol) Day 5: Post-rehydration testing (after the rehydration protocol)",[131],"Aging",[133,134],"rehydration","hypohydration","2025-09-11",{"date":137,"type":138},"2025-09-17","ACTUAL",{"date":140,"type":138},"2025-07-15",{"date":142,"type":126},"2026-12",{"name":5,"class":6},1]