[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"health-study-detail:100619214":3},{"organization":4,"outcomesModule":7,"designInfo":174,"detailedDescription":179,"studyPopulation":173,"armGroups":180,"interventions":193,"overallOfficials":173,"centralContacts":201,"locations":208,"responsibleParty":231,"collaborators":173,"id":235,"slug":173,"hasResults":236,"nctId":237,"briefTitle":238,"officialTitle":239,"acronym":173,"eligibilityCriteria":240,"healthyVolunteers":241,"sex":242,"minAge":243,"maxAge":244,"enrollmentInfo":245,"targetDuration":173,"studyType":248,"phases":249,"briefSummary":251,"conditions":252,"keywords":254,"overallStatus":211,"whyStopped":173,"lastUpdateSubmitDate":259,"lastUpdatePostDateStruct":260,"startDateStruct":263,"completionDateStruct":265,"leadSponsor":267,"locationsCount":268},{"fullName":5,"class":6},"University of Thessaly","OTHER",{"primaryOutcomes":8,"secondaryOutcomes":82,"otherOutcomes":173},[9,13,16,19,22,25,28,31,34,37,40,43,46,49,52,55,58,61,64,67,70,73,76,79],{"measure":10,"description":11,"timeFrame":12},"Change in mitochondrial size","Mitochondrial size will be measured using transmission electron microscope","At baseline and at 6 months",{"measure":14,"description":15,"timeFrame":12},"Change in mitochondrial density","Mitochondrial density will be measured using transmission electron microscope",{"measure":17,"description":18,"timeFrame":12},"Change in mitochondrial count","Mitochondrial count will be determined using transmission electron microscope",{"measure":20,"description":21,"timeFrame":12},"Change in mitochondrial distribution","Mitochondrial distribution will be determined using transmission electron microscope",{"measure":23,"description":24,"timeFrame":12},"Change in maximum oxygen consumption (VO2max)","Maximum oxygen consumption (VO2max) will be assessed during a cardiopulmonary exercise testing by using a portable indirect calorimetry system",{"measure":26,"description":27,"timeFrame":12},"Change in muscle fiber cross-sectional area","Muscle fiber cross-sectional area (μm2) will be measured using immunohistochemical staining for myosin heavy chain",{"measure":29,"description":30,"timeFrame":12},"Change in PAX7+ satellite cells count","PAX7+ satellite cells will be determined using immunohistochemistry techniques.",{"measure":32,"description":33,"timeFrame":12},"Change in total protein content","Total protein content (total RNA) will be determined in skeletal muscle tissue using real time quantitative-Polymerase Chain Reaction (q-PCR) technique",{"measure":35,"description":36,"timeFrame":12},"Change in myonuclei content","Myonuclei content will be determined in skeletal muscle tissue using immunohistochemistry techniques",{"measure":38,"description":39,"timeFrame":12},"Change in peroxisome proliferator-activated receptor-gamma coactivator -1a (PGC-1a) expression","PGC-1a expression in skeletal muscle tissue will be assessed using immunoblotting techniques.",{"measure":41,"description":42,"timeFrame":12},"Change in Krebs cycle (TCA cycle) enzymes activity","Krebs cycle enzymes activity will be determined using the Seahorse XF Analyzer",{"measure":44,"description":45,"timeFrame":12},"Change in protein expression of respiratory chain complexes","Protein expression of respiratory chain complexes will be determined using immunoblotting techniques",{"measure":47,"description":48,"timeFrame":12},"Change in cytochrome C oxidase amount and expression","Cytochrome C oxidase amount and expression will be assessed using immunohistochemistry and immunoblotting techniques",{"measure":50,"description":51,"timeFrame":12},"Change in ATP synthase amount and expression","ATP synthase amount and expression will be assessed using immunohistochemistry and immunoblotting techniques",{"measure":53,"description":54,"timeFrame":12},"Change in citrate synthase amount and expression","Citrate synthase amount and expression will be assessed using immunohistochemistry and immunoblotting techniques",{"measure":56,"description":57,"timeFrame":12},"Change in succinate dehydrogenase amount and expression","Succinate dehydrogenase amount and expression will be assessed using immunohistochemistry and immunoblotting techniques",{"measure":59,"description":60,"timeFrame":12},"Change in NADH dehydrogenase amount and expression","NADH dehydrogenase amount and expression will be assessed using immunohistochemistry and immunoblotting techniques",{"measure":62,"description":63,"timeFrame":12},"Change in mitochondrial oxygen consumption rate","Mitochondrial oxygen consumption rate will be determined using the Seahorse XF Analyzer",{"measure":65,"description":66,"timeFrame":12},"Change in spare respiratory capacity","Spare respiratory capacity will be determined using the Seahorse XF Analyzer",{"measure":68,"description":69,"timeFrame":12},"Change in mitochondrial maximal respiration","Maximal mitochondrial respiration will be determined using the Seahorse XF Analyzer",{"measure":71,"description":72,"timeFrame":12},"Change in mitochondrial basal respiration","Mitochondrial basal respiration will be determined using the Seahorse XF Analyzer",{"measure":74,"description":75,"timeFrame":12},"Change in non-mitochondrial respiration","Non-mitochondrial respiration will be determined using the Seahorse XF Analyzer",{"measure":77,"description":78,"timeFrame":12},"Change in body fat percentage","Body fat percentage will be assessed using dual energy x-ray absorptiometry (DEXA)",{"measure":80,"description":81,"timeFrame":12},"Change in diastolic arterial pressure","Diastolic blood pressure will be measured using a sphygmomanometer",[83,86,89,92,95,98,101,104,107,110,113,116,119,122,125,128,131,134,137,140,143,146,149,152,155,158,161,164,167,170],{"measure":84,"description":85,"timeFrame":12},"Change in density and distribution of capillaries","Capillarization will be determined using immunohistochemistry techniques",{"measure":87,"description":88,"timeFrame":12},"Change in skeletal muscle fiber typing","Fiber typing will be determined using immunohistochemistry techniques",{"measure":90,"description":91,"timeFrame":12},"Change in GLUT-4 protein expression","GLUT-4 protein expression will be assessed using immunoblotting techniques",{"measure":93,"description":94,"timeFrame":12},"Change reduced glutathione content in skeletal muscle cells","Reduced glutathione content will be determined spectrophotometrically",{"measure":96,"description":97,"timeFrame":12},"Change in glutathione peroxidase activity in skeletal muscle cells","Glutathione peroxidase activity will be determined spectrophotometrically",{"measure":99,"description":100,"timeFrame":12},"Change in glutathione reductase activity in skeletal muscle cells","Glutathione reductase activity will be determined spectrophotometrically",{"measure":102,"description":103,"timeFrame":12},"Change in superoxide dismutase activity in skeletal muscle cells","Superoxide dismutase activity will be determined spectrophotometrically",{"measure":105,"description":106,"timeFrame":12},"Change in fasting glucose levels","Fasting glucose levels will be measured on an automated clinical chemistry analyzer",{"measure":108,"description":109,"timeFrame":12},"Change in fasting insulin levels","Fasting insulin levels will be measured on an automated clinical chemistry analyzer",{"measure":111,"description":112,"timeFrame":12},"Change in glycosylated hemoglobin levels","Glycosylated hemoglobin levels will be measured on an automated clinical chemistry analyzer",{"measure":114,"description":115,"timeFrame":12},"Change in high-density lipoprotein (HDL) levels","HDL will be measured on an automated clinical chemistry analyzer",{"measure":117,"description":118,"timeFrame":12},"Change in low-density lipoprotein (LDL) levels","LDL will be measured on an automated clinical chemistry analyzer",{"measure":120,"description":121,"timeFrame":12},"Change in total cholesterol levels","Total cholesterol will be measured on an automated clinical chemistry analyzer",{"measure":123,"description":124,"timeFrame":12},"Change in triglyceride levels","Triglycerides will be measured on an automated clinical chemistry analyzer",{"measure":126,"description":127,"timeFrame":12},"Change in general blood count","General blood count will be measured on a hematology analyzer",{"measure":129,"description":130,"timeFrame":12},"Change in erythrocyte reduced glutathione (GSH) levels","Erythrocyte GSH levels will be determined spectrophotometrically",{"measure":132,"description":133,"timeFrame":12},"Change in erythrocyte oxidized glutathione (GSSG) levels","Erythrocyte GSSG levels will be determined spectrophotometrically",{"measure":135,"description":136,"timeFrame":12},"Change in myostatin expression","Myostatin expression will be assessed using immunoblotting techniques",{"measure":138,"description":139,"timeFrame":12},"Change cortisol concentration","Blood cortisol concentration will be assessed using immunoassays (ELISA)",{"measure":141,"description":142,"timeFrame":12},"Change in testosterone concentration","Blood testosterone concentration will be assessed using immunoassays (ELISA)",{"measure":144,"description":145,"timeFrame":12},"Change in growth hormone concentration","Blood growth hormone concentration will be assessed using immunoassays (ELISA)",{"measure":147,"description":148,"timeFrame":12},"Change in insulin-like growth factor-1 (IGF-1) concentration","Blood IGF-1 concentration will be assessed using immunoassays (ELISA)",{"measure":150,"description":151,"timeFrame":12},"Change in body mass","Body mass will be measured on a beam scale",{"measure":153,"description":154,"timeFrame":12},"Change in bone density","Bone density will be assessed using dual energy x-ray absorptiometry (DEXA)",{"measure":156,"description":157,"timeFrame":12},"Change in fat-free mass","Fat-free mass will be assessed using dual energy x-ray absorptiometry (DEXA)",{"measure":159,"description":160,"timeFrame":12},"Change in waist circumference","Waist circumference will be measured using a Gullick II tape",{"measure":162,"description":163,"timeFrame":12},"Change in hip circumference","Hip circumference will be measured using a Gullick II tape",{"measure":165,"description":166,"timeFrame":12},"Change in resting heart rate","Heart rate will be measured using a heart rate monitor",{"measure":168,"description":169,"timeFrame":12},"Change in resting metabolic rate (RMR)","RMR will be measured using indirect calorimetry",{"measure":171,"description":172,"timeFrame":12},"Change in systolic arterial pressure","Systolic blood pressure will be measured using a sphygmomanometer",null,{"allocation":175,"interventionModel":176,"interventionModelDescription":173,"primaryPurpose":6,"observationalModel":173,"timePerspective":173,"maskingInfo":177},"RANDOMIZED","PARALLEL",{"masking":178,"maskingDescription":173,"whoMasked":173},"NONE","A total number of 30 adults (both males and females) aged 30-50, meeting the inclusion criteria, will be enrolled in this study. Participants will be randomly assigned to either (i) a Control group or (ii) an Intervention group. The Intervention group will participate in three hybrid-type HIIT sessions per week over a 6-month period while receiving a balanced diet. The Control group will receive a balanced diet over the 6-month period but will not participate in exercise training. At baseline and 6 months, both groups will undergo assessment of their anthropometric profile, body composition, resting metabolic rate, muscle strength and cardiorespiratory capacity and provide resting blood and skeletal muscle samples.",[181,187],{"label":182,"type":183,"description":184,"interventionNames":185},"Intervention group","EXPERIMENTAL","Participants in this arm will participate in three hybrid-type HIIT sessions per week over a 6-month period while receiving a balanced diet",[186],"Other: Exercise training",{"label":188,"type":189,"description":190,"interventionNames":191},"Control group","ACTIVE_COMPARATOR","Participants in this arm will receive a balanced diet over the 6-month period but will not participate in exercise training",[192],"Other: Control group",[194,198],{"type":6,"name":195,"description":196,"armGroupLabels":197,"otherNames":173},"Exercise training","Participants will perform a six-month hybrid training program while receiving a balanced diet. The periodization of hybrid training intervention will consist of three 2-month phases of gradually increased exercise intensity and volume. In every training will participate 5-8 individuals. The training will contain 6-12 different exercises (stations), depending on the phase of the intervention, which will be executed in a circuit for a total of 2-3 rounds, with 2-3 minutes of rest period between sets (depending on the phase). The exercise execution will last 20-45 seconds, and the rest between them will last 30-60 seconds (depending on the phase), while the exercise intensity will range from 75 to 85% of maximal heart rate. The stations of hybrid training will contain multi-joint exercises or neuromuscular activation exercises using either body weight resistance or portable equipment.",[182],{"type":6,"name":188,"description":199,"armGroupLabels":200,"otherNames":173},"Participants will receive a balanced diet but will not participate in any type of exercise training over a six month period.",[188],[202],{"name":203,"role":204,"phone":205,"phoneExt":206,"email":207},"Ioannis G Fatouros, Professor","CONTACT","24310 47047","0030","ifatouros@pe.uth.gr",[209],{"facility":210,"status":211,"city":212,"state":213,"zip":214,"country":215,"countryCode":216,"cosmosGeoPoint":217,"geoPoint":222,"contacts":223},"Department of Physical Education and Sport Science Trikala, University of Thessaly","RECRUITING","Trikala","Thessaly","42100","Greece","GR",{"type":218,"coordinates":219},"Point",[220,221],21.76837,39.55493,{"lat":221,"lon":220},[224,227],{"name":203,"role":204,"phone":225,"phoneExt":173,"email":226},"+30 2431047047","ifatouros@uth.gr",{"name":228,"role":204,"phone":229,"phoneExt":173,"email":230},"Athanasios Z Jamurtas, Professor","+30 2431047054","ajamurt@uth.gr",{"type":232,"investigatorFullName":233,"investigatorTitle":234,"investigatorAffiliation":5,"oldNameTitle":173,"oldOrganization":173},"PRINCIPAL_INVESTIGATOR","Ioannis G. Fatouros","Professor","100619214",false,"NCT07341711","Long-term Adaptations of Skeletal Muscle After Hybrid Training","Long-term Adaptations of Skeletal Muscle in Overweight and Obese Individuals After Hybrid Training","Inclusion Criteria:\n\n* BMI: \\>25 kg\u002Fm2 and \\\u003C40 kg\u002Fm2\n* Untrained individuals (abstain \\>1 year from exercise training)\n* No dietary intervention over the last 6 months preceding the study\n* Low cardiorespiratory fitness level (VO2max: \\\u003C45 ml\u002Fkg\u002Fmin)\n* No use of any medication, dietary supplements\n* Low risk for cardiovascular disease\n* A weight loss \\\u003C10% over the last 6 months preceding the study\n* Free of non-communicable diseases (excluding metabolic syndrome)\n\nExclusion Criteria:\n\n* Low participation rate (\\\u003C80% completion of the exercise training sessions)\n* Unbalanced diet\n* Participation in additional exercise training regimes\n* Cosumption of anti-inflammatory of pain relief medication",true,"ALL","30 Years","50 Years",{"count":246,"type":247},30,"ESTIMATED","INTERVENTIONAL",[250],"NA","Obesity is a major challenge for public health and renders it imperative to reduce its prevalence. High intensity interval training (HIIT) is a form of exercise training that can efficiently induce weight loss in adults with overweight or obesity, even in the absence of dietary intake manipulation. Hybrid type training represents a form of HIIT, that incorporates both cardiorespiratory and musculoskeletal stimuli, by combining multiple types of exercise into a circuit-type, interval style workout. Recent evidence suggests that long-term participation in hybrid HIIT results in significant health-related benefits. However, the molecular mechanisms driving the chronic effects of hybrid HIIT on cardiometabolic and musculoskeletal health remains to be elucidated.",[253],"Obesity & Overweight",[255,256,257,258],"obesity","overweight","high-intensity interval training","skeletal muscle","2026-06-09",{"date":261,"type":262},"2026-06-12","ACTUAL",{"date":264,"type":262},"2026-01-08",{"date":266,"type":247},"2026-12-20",{"name":5,"class":6},1]